In vivo enzymatic assay of carnitine acetyl transferase and acetylCoA synthetase using hyperpolarized acetate
نویسندگان
چکیده
Jessica A M Bastiaansen, Tian Cheng, Mor Mishkovsky, Arnaud Comment, and Rolf Gruetter Laboratory of Functional and Metabolic Imaging, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland, Department of Radiology, Universite de Lausanne, Lausanne, Switzerland, Institute of Physics of Biological Systems, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland, Department of Radiology, Universite de Lausanne and Geneva, Lausanne and Geneva, Switzerland
منابع مشابه
Tissue-specific short chain fatty acid metabolism and slow metabolic recovery after ischemia from hyperpolarized NMR in vivo.
Mechanistic details of mammalian metabolism in vivo and dynamic metabolic changes in intact organisms are difficult to monitor because of the lack of spatial, chemical, or temporal resolution when applying traditional analytical tools. These limitations can be addressed by sensitivity enhancement technology for fast in vivo NMR assays of enzymatic fluxes in tissues of interest. We apply this me...
متن کاملMetabolic flux analysis of acetylcarnitine turnover and mitochondrial oxidation of [2-13C]acetate in rat skeletal muscle in vivo measured by 13C MRS
INTRODUCTION: Acetylcarnitine is a necessary intermediate in the mitochondrial oxidation of acetate, since it transports the acetyl moiety across the mitochondrial membrane [1]. It has only been observed using hyperpolarized methods [2,3] or with localized polarization transfer sequences [5]. Glutamate has been used as an indicator of citric acid cycle (TCA) fluxes following [2-C]acetate infusi...
متن کاملIn vivo enzymatic activity of acetylCoA synthetase in skeletal muscle revealed by (13)C turnover from hyperpolarized [1-(13)C]acetate to [1-(13)C]acetylcarnitine.
BACKGROUND Acetate metabolism in skeletal muscle is regulated by acetylCoA synthetase (ACS). The main function of ACS is to provide cells with acetylCoA, a key molecule for numerous metabolic pathways including fatty acid and cholesterol synthesis and the Krebs cycle. METHODS Hyperpolarized [1-(13)C]acetate prepared via dissolution dynamic nuclear polarization was injected intravenously at di...
متن کاملHyperpolarized [1-13C]acetylcarnitine as a tracer for cardiac metabolism
Introduction: Acetylcarnitine (ALCAR) is known to facilitate the influx and efflux of acetylgroups across the mitochondrial membrane and buffers an excess in acetylCoA in both cytosolic and mitochondrial compartments [1]. Acetylcarnitine is a necessary intermediate for mitochondrial acetate metabolism [2] and has been shown to easily cross the blood brain barrier and act as a neuroprotective ag...
متن کاملAcetate trafficking in the heart: carnitine acyltransferases matter
Dear Editor, The use of hyperpolarized C magnetic resonance to study intermediary metabolism is increasing rapidly, since it allows in vivo noninvasive investigation of dynamic metabolic and physiological processes that were previously experimentally inaccessible. A recent study published in the Journal by Koellish and colleagues used the technique to address the fate of hyperpolarized Clabeled...
متن کامل